Background: Spuriously high level of glycosylated hemoglobin has been observed in non-diabetic hypothyroid patients in recent studies. Aims:To investigate the impact of thyroid hormones on Serum HbA1c levels in an urban female hypothyroid nondiabetic population in Eastern India. Materials & methods: This present observational cross sectional study was conducted on 200 hypothyroid subjects in Burdwan Medical College in a period of 12 months after taking Institutional Ethical Clearance and informed consent of the subjects.100 controls were also included for the study. Serum TSH, FT4, HbA1c and Fasting plasma glucose levels were estimated. The subjects were age and blood glucose matched. The computer software SPSS, version 16.0 was used for analyzing data. Results: Significant difference was observed between control and hypothyroid subjects for mean TSH(P<0.0001), mean FT4(P<0.0001) and mean HbA1c (P<0.0001). No significant difference was observed for mean age (Age in years: 30.82±6.55 vs. 31.54 ±6.68; P value: 0.376) and fasting plasma glucose (83.97± 6.24 vs84.65±6.00, p=0.362) between control and hypothyroid subjects. Serum HbA1c level was positively correlated with serum TSH (r=0.684, P<0.00001) and was negatively correlated with Sr. FT4 (r= - 0.495, P<0.00001). Conclusion: Serum HbA1c levels were significantly higher in Hypothyroid nondiabetic females compared to controls. There is a positive correlation between HbA1c and TSH. In hypothyroid individual assessment of HbA1c levels may be considered as an important parameter for diagnosis of diabetic or prediabetes state.