Previous studies have demonstrated the type of chromosomal abnormalities found among couples with recurrent pregnancy loss. But a majority of the couples show a normal karyotype. The present study was undertaken to inquire the nature of cytogenetic abnormalities by routine karyotyping and c-banding whenever required, among couples with recurrent pregnancy loss and to compare it with fertile couples. Among couples, women with the history of two or more than two spontaneous abortions ≤ 24 weeks of gestation were included and couples with the history of Diabetes mellitus, thyroid disorders and hypertension, etc. were excluded. The lymphocytes were cultured as per the standard protocol and metaphase spreads stained by GTG banding technique. The presence of chromosomal aberrations was analyzed by semi-automated karyotyping software (Ikaros). In the present study, abnormal karyotypes were found in 3 cases. All the controls had a normal karyotype.