Contains accurate mass, HPLC retention time, peptide sequence, glycan structure and mass spectra for over 400 glycopeptides from elastase digestion of SARS-COV2 Spike antigen and receptor binding domain. The database is used as part of the Mass-Retention Time Fingerprinting method to characterise Spike glycans as described in the BioRxiv preprint “Identification, Mapping and Relative Quantitation of SARS-Cov2 Spike Glycopeptides by Mass-Retention Time Fingerprinting”