This supporting information provides all input data of the model, the assumptions made and the literature and database references for the publication Environmental benefits of large-scale second-generation bioethanol production in the EU: An integrated supply chain network optimization and Life Cycle Assessment approache. The environmental data is based on life cycle assessments, with full information on the life cycle inventory and the results of the life cycle impact assessment based on the ReCiPe method. It also includes detailed results for all objective functions in all scenarios (optimization of 18 midpoints, 3 endpoints, and economic optimization in 5 tax scenarios and 2 feedstock scenarios), and detailed results of the sensitivity analysis and Pareto optimization.