Introduction: The installation of a paper patch is a routine procedure in predicting hearing repair before performing a myringoplasty operation on a tympanic membrane that has rupture or perforation that fails to close. However, paper patches have various deficiencies such as being inelastic, non-transparent and easily torn. The nata de coco patch (NCP) which is a natural biopolymer formed on coconut media with the help of acetobacterxylinum with unique characteristics such as being alike to human skin, elastic, transparent and not easily torn is offered to be a prior and intra myringoplasty patch material replacing the paper patch. Objective: To determine the effectiveness of NCP in accelerating the healing / closure of tympanic membrane perforation due to infection or trauma compared with paper patch. Method: This study was an experimental study with complete random sampling of 42 ruptured tympanic membranes of chicken (laying hen) divided into 3 groups: PNC + group, paper patch and control (spontaneous healing). Result: healing of tympanic membrane perforation by using PNC has started on day 4 of 56.8% and total closure on day 6th, meanwhile on the paper patch group only 7.2% closed by day 4th and total sample closure occurred on day7th. In the control group 21.3% began closing on day 5 and total sample closure occurred on day 8. Conclusion: In vivo NCP proved to accelerate the healing and closure of tympanic membrane perforation more quickly than either the paper patch or control group. We recommend NCP to be a platform material for myringoplasty in humans.