This high temporal resolution dataset of a strawberry plant was captured in two experiments, each lasting 100h. On leaf was inserted into a leaf chamber of the LI-6400XT gas exchange system, capturing information on transpiration, photosynthesis and stomatal conductance. Environmental characteristics are also captured at canopy height. These experiments were conducted in a growth chamber at ILVO (Melle, Belgium) and only covered conditions that did not result in stress in the plant. As such, this dataset attempts to capture subtle dynamic variation in the plant.