2-substituted derivatives of indane-1,3-diones have shown many activities. Chalcones possess a number of biological and pharmacological activities such as antibacterial, antifungal, antimalarial, anti-inflammatory, anticancer, anti-tubercular, and antioxidant activities etc. The synthesis of chalcone compounds incorporating with heterocycles became great importance in medicinal chemistry. Literature survey revealed that Chalcones and Pyrimidine derivatives possess a broad spectrum of biological activities like antimicrobial, anti-inflammatory, antimalarial, antitubercular, anticancer, antidepressant and antamoebic etc.Hence an attempt is made to synthesize some new chalcones from 2- acetyl-indane- 1,3- dione with various substituted aromatic/ heteroaromatic aldehydes by the Claisen – Schmidt condensation. The resulted chalcones have been converted into their 2-amino pyrimidine derivatives by reaction with guanidine hydrochloride. The resulted compounds were identified by physical and spectral methods and were also screened for their antibacterial and antifungal activities based on the reported literature. 2-substituted derivatives of indane-1,3-diones have shown many activities. Chalcones possess a number of biological and pharmacological activities such as antibacterial, antifungal, antimalarial, anti-inflammatory, anticancer, anti-tubercular, and antioxidant activities etc. The synthesis of chalcone compounds incorporating with heterocycles became great importance in medicinal chemistry. Literature survey revealed that Chalcones and Pyrimidine derivatives possess a broad spectrum of biological activities like antimicrobial, anti-inflammatory, antimalarial, antitubercular, anticancer, antidepressant and antamoebic etc.Hence an attempt is made to synthesize some new chalcones from 2- acetyl-indane- 1,3- dione with various substituted aromatic/ heteroaromatic aldehydes by the Claisen – Schmidt condensation. The resulted chalcones have been converted into their 2-amino pyrimidine derivatives by reaction with guanidine hydrochloride. The resulted compounds were identified by physical and spectral methods and were also screened for their antibacterial and antifungal activities based on the reported literature.