Practice of traditional medicine varies greatly from country to country, and the variation is seriously influenced by refugees. Refugees are facing significant barriers in accessing healthcare services. This study assessed knowledge, attitude and practice of south Sudanese refugees in Ethiopia towards traditional medicine. A community based cross-sectional study design was conducted on total of 151 randomly selected household from south Sudanese refugees in Addis Ababa, Ethiopia. Structured interviewer administered questionnaire was used to collect data. Chi square test was used to determine association between variables. From the total of 151 participants, 84 (55.6%) were males. About 141 (96.7%) of the respondents know traditional medicine, and 120 (79.5%) of the respondents think as there were diseases which were not cured by modern medicine. Thus, 102 (67.5%) of respondents encourage others to use traditional medicine. Gender (p=0.020), duration of stay (p=0.008), marital status (p=0.000), family size (p=0.041), occupational status (p=0.000), and educational status (p=0.000) were significantly associated with practice of traditional medicine among refugees which lost their home. In conclusion, majority of respondents know traditional medicine especially, herbal type and use it because of their belief that herbals are natural thus encourage others practices. Moreover, practice of traditional medicine was significantly associated gender, duration of stay, marital status, family size, occupational status and educational status. Further study on standardization of herbal medicine is paramount to ensure its safety.