Labeling and tracking of immune cells in ex vivo human skin

Authors: Dijkgraaf, Feline; Toebes, Mireille; Hoogenboezem, Mark; Mertz, Marjolijn; Vredevoogd, David W.; Matos, Tiago R.; Teunissen, Marcel B. M.; +2 Authors

doi: 10.5281/zenodo.3843094 , 10.5281/zenodo.3843093

Labeling and tracking of immune cells in ex vivo human skin

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Abstract

Example imaging datasets linked to the publication 'Labeling and tracking of immune cells in ex vivo human skin' (doi: 10.1038/s41596-020-00435-8). Additional information filenames: - m = ex vivo murine skin - h = ex vivo human skin - mCD8= anti-murine CD8-AF594 nanobody staining (red) - hCD8= anti-human CD8-AF594 nanobody staining (red) - Hoechst= Hoechst 33342 staining (grey) - CD1a= anti-hCD1a-AF488 staining (green) - CD103= anti-hCD103-AF488 staining (green) - SHG = second harmonic generation (cyan) Imaris x64 v9.2.0.

This work was supported by ERC AdG Life-His-T (to T.N.S.) and an EADV Research Fellowship (to T.R.M.).

Keywords

Multi-photon imaging, CD8 T cells, Human skin, Ex vivo

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