Urinary tract Infection has become on the top of the list of common infectious diseases worldwide especially in Bangladesh. This study attempted to emphasize the prevalence of UTI patients affected by uropathogenic strains admitted in Brahmanbaria Medical College, Bangladesh. The study was conducted among 150 patients of different ages and sex from November, 2019 to January, 2020 through conventional cultural, biochemical methods as well as disk diffusion methods (Kirby-Bauer methods) to determine the uropathogens and their antibiotic susceptibility pattern. Among 150 samples, 50 were male, 50 were female and the rest of the samples were children. About 33.33% cases were UTI positive among which 48% female patients were UTI positive. Most of the UTI were caused by Escherichia coli (48%). Moreover, Enterobacter spp, Pseudomonas aeroginosa, Staphylococcus aures, Proteus vulguris, Citrobacter spp, Staphylococcus saprophyticus were also estimated. The pathogens showed 100% resistance against Amoxicillin and the sensitivity (84%) was recorded against Meropename. Nonetheless, Nitrofurantion, Amikacin and Gentamicin were also effective against the isolates. The significant numbers of pathogens were found to be resistant against more than one antibiotics denoted as Multidrug Resistant (MDR) strain which might be hinder the proper treatment of the patients.