Objectives:Bauhinia scandens Linn,and Senna sopherahas been locally known as monkey ladders and Kalkasunda in Bangladesh.They have several significant traditional uses as a source of the medicinal agent. This study is to implement the thrombolytic and cytotoxic activity of n-Hexane fraction of Bauhinia scandensstems and Senna sopherastems (NHBS, and NHSS). Methods: In this study, in vitro clot lysis method and brine shrimp lethality method were implemented to check the thrombolytic and cytotoxic potentiality of NHBS and NHSS. Results: In the thrombolytic test, NHBS (24.52 ? 2.86%; P< 0.001) attained a significant percentage of clot lysis but NHSS (16.59 ? 4.07%; P< 0.001) showed moderate clot lysis potentiality in human blood. Again, the LD50 value of NHBS and NHSS was seen at 92.55 ?g/mL and 338.54 ?g/mL of doses respectively in brine shrimp lethality bioassay. Conclusion: Present results suggested, NHBS showed comparatively potent thrombolytic activity than NHSS, besides, a comparative study ofthe LD50 value of NHBS and NHSS suggested that the NHBS has strong anti-tumor potentiality other than NHSS.