This repository contains the datasets for benchmarking of a two-pass alignment method for long reads using the snakemake pipeline which can be found on github at https://github.com/bartongroup/two_pass_alignment_pipeline The datasets contained here are genuine nanopore DRS datasets re-basecalled from Parker et al 2020 and Workman et al. 2019. There are also simulated alignments which are each created using the error profiles of a single genuine replicate .