These Guidelines are intended to deal with this complex set of issues, starting from a simple question: how can RRI and OS be effectively embedded in research organisations? They're not intended to offer ready-made solutions to this problem, since readymade solutions simply do not exist. Rather, their main aim is to propose a pathway – in which the users of the guidelines will necessarily play a proactive and creative role – for activating institutional change processes towards RRI and OS in their organisation in a way that is as feasible, sustainable and useful as possible. The Guidelines can be useful for all those interested in promoting RRI and OS at any level, including, e.g., policymakers, science centres, technology developers or private firms. However, as can be easily understood, the main targets of these Guidelines are European research funding and performing organisations of any kind (universities, research labs, funding agencies, governmental research agencies, etc.) since they are increasingly asked to introduce institutional changes towards RRI and OS and are exposed the most to the transformations affecting science. The Guidelines are one the main products of the project “Fostering Improved Training Tools for Responsible Research and Innovation - FIT4RRI”, co-funded by the EU DG Research and Innovation under Horizon 2020 and coordinated by Sapienza University of Rome.