Material and Methods: "HBLAK cells were harvested by accutase after routine cultivation in T25 flasks for two days. Cell viability and cell number analysis were performed via trypan blue staining in a Neubauer counting chamber. A total of 20,000 cells were used as input for the single-cell droplet libraries generation on the 10× Chromium Controller system utilizing the Chromium Single Cell 3 Reagent Kit v3 according to manufacturer's instructions. Sequencing was carried out on a HiSeq 3000 system (Illumina Inc. San Diego, CA, USA) with a mean sequencing depth of ~40,000 reads/cell. Raw sequencing data were processed using the 10× Genomics CellRanger software (v3.1). Raw BCL-files were demultiplexed and processed to Fastq-files using the CellRanger mkfastq pipeline. Alignment of reads to the GRCh38 genome and UMI counting was performed via the CellRanger count pipeline to generate a gene-barcode matrix [filtered_feature_bc_matrix.zip]."

{"references": ["Lang, A.; Whongsiri, P.; Yilmaz, M.; Lautwein, T.; Petzsch, P.; Greife, A.; G\u00fcnes, C.; K\u00f6hrer, K.; Niegisch, G.; Hoffmann, M.; Schulz, W.A. Knockdown of UTX/KDM6A Enriches Precursor Cell Populations in Urothelial Cell Cultures and Cell Lines. Cancers 2020, 12, 1023."]}

This data set of the single cell sequencing experiment of the urothelial cell line HBLAK belongs to the publication: "Knockdown of UTX/KDM6A Enriches Precursor Cell Populations in Urothelial Cell Cultures and Cell Lines" Cancers 2020, 12(4), 1023; https://doi.org/10.3390/cancers12041023.