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53 marker MELC Run in human tonsil. Each image depicts the same field of view, sequentially stained with the depicted fluorescence-labelled antibodies. Images contain 2048 x 2048 pixels and are generated using an inverted wide-field fluorescence microscope with a 20x objective, a lateral resolution of 325 nm and an axial resolution above 5 µm. Images have not been normalized and intensities have not been adjusted.
This study was supported by funding from the Deutsche Forschungsgemeinschaft, (SPP1937, HA5354/8-2 to A.E.H.) The work was supported by an "Adding 3R Value" grant of the Charité - Universitätsmedizin, Berlin.
immunofluorescence-based histology, tonsil, highly multiplexed histology, human colon, MELC, hihgly multiplexed histology
immunofluorescence-based histology, tonsil, highly multiplexed histology, human colon, MELC, hihgly multiplexed histology
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