Background:Medico-legal amenities have reached across the world. The purpose of medico-legal services is to provide better care to victims and to collectmedico-legal evidence.1 It has been a challenge for nurses to take liability with judicial system to magnify the means applicable for patients with obligation related injuries, crime casualty and somebody suspicious under police custody.2This signifies advanced aspects in view of comprehensive approach to accountability matters involving patient care in the emergency care setting. Aim: To determine the Effectiveness of structured guidelines on the knowledgeof nursing personnel regarding management of medico-legal cases. Material and Method:Quasi Experimental one group pretest posttest design with Quantitative approach was adopted. Total 34 nursing personnelwere selected through total Enumerative sampling. Information was gathered from 34 nursing personnel by means of structured knowledge questionnaire. Result:Outcomes reveals that Mean posttest knowledge score was significantly higher 18.02 ? 1.02 than that of mean pretest knowledge score 14.2 ? 2.20 with calculated t value 9.44. Conclusion:Results of the study conclude that in-service education program was effective in boosting the knowledge of nursing personnel on management of medico-legal cases.