The present study was designed to assess the comparative bio-equivalence of Estreptovall and Nilestrept in healthy broiler chickens after IM injection of both products in a dose of 25 mg streptomycin base/kg.b.wt. Twenty four broiler chickens were divided into two groups. The first group was designed to study the pharmacokinetics of Estreptovall, while the 2nd group was designed to study the pharmacokinetics of Nilestrept. Each broiler chicken in both groups was IM injected with 25 mg streptomycin base/kg.b.wt. Blood samples were obtained from the wing vein and collected immediately before and at 0.08, 0.16, 0.25, 0.5, 1, 2, 4, 6, 8, 12 and 24 hours after a single IM injection. The disposition kinetics of Estreptovall and Nilestrept following IM injection of 25 mg streptomycin base/kg.b.wt. revealed that the maximum blood concentration [Cmax] were 25.75 and 23.84 μg/ml and attained at [tmax] of 2.50 and 2.51 hours, respectively. In conclusion: Nilestrept is bioequivalent to Estreptovallsince the ratios of Cmax, AUC0-24 andAUC0-∞ (T/R) were 0.93, 0.92 and 0.92 respectively. These are within the bioequivalence acceptance range. Estreptovall and Nilestreptare therefore bioequivalent and interchangeable.