Lantana camara is a noxious and invasive weed from family Verbenaceae, native to tropical America, but now widespread in many countries. However, there has been little research on its autofluorescence producing flurophores that play very crucial biological role inside the cells. In the present study, the fluorescence based spectral fingerprint analysis of leave extract was studied in Lantana camara. A peculiar pattern of fingerprints were observed, indicating accumulation of metabolites and enzymes in cells. Further, as per the excitation λ, different fingerprints were detected. Based on these findings possible role of spectral fingerprints in correlation with accumulation pattern of metabolites was discussed.