Samples were collected at or in the vicinity of Station ALOHA on 24 different cruises. On each cruise, rates of 3H-Leu incorporation into protein were measured. Seawater samples for measurements of production were collected from pre-dawn vertical hydrocasts at 6 discrete depths (5, 25, 45, 75, 100, 125 m) using polyvinyl chloride sampling bottles affixed to a rosette sampler. Polyethylene amber bottles (125 ml capacity) were subsampled from the CTD rosette bottles, and duplicate acid-cleaned 12 ml polycarbonate centrifuge tubes were filled from each depth. Each polycarbonate tube was inoculated with 20 nmol L-1 (final concentration) 3,4,5-3H-leucine. An additional 1.5 ml per depth was subsampled into 2 ml microcentrifuge tubes containing 100 µl of 100% (w/v) ice-cold trichloroacetic acid (TCA) to serve as a killed blank. The polycarbonate sample tubes were capped and incubated in situ over the photoperiod to measure rates of 3H-Leu incorporation in both dark (through use of black cloth bags) and light on a free-drifting array. At the end of the photoperiod, triplicate 1.5 ml subsamples were removed from each tube and added to 2 ml microcentrifuge tubes containing 100 µl of 100% ice-cold TCA; these tubes were stored frozen until analysis. Samples were processed following a modified method of the microcentrifuge method.

{"references": ["Viviani, Donn & Church, Matthew. (2017). Decoupling between bacterial production and primary production over multiple time scales in the North Pacific Subtropical Gyre. Deep Sea Research Part I: Oceanographic Research Papers. 121. 10.1016/j.dsr.2017.01.006."]}