The HAP Nanoparticles were produced from Sol-Gel method by adding 1M of calcium hydroxide, 0.6M of orthophosphoric Acid were prepared using papaya (Carica papaya) leaf and Indian nettle (acalypha indica) leaf extract as the solvents. Sol-Gel method was not sensitive regarding the preparation conditions, easy to operate and implement, which controlled the structure, morphology, and particle size of nanomaterials. The HAP materials synthesized by sol-gel process were efficient to improve the contact and stability at the artificial/natural bone interfaces in both in vitro and in vivo environment.Hydroxyapatite was the dominant inorganic phase in natural bone. Synthetic hydroxyapatite particles, films, coatings, fibers and porous skeletons were used extensively in various biomedical applications. The presence of HAP nanoparticles were studied by various analytical techniques such as UV, XRD, FTIR and SEM. HAP had excellent bioactivity and biocompatibility along with strong antimicrobial activity that make them as potential materials for tissue engineering, orthopaedic and dental applications.