The purpose of this study is to investigate the relationship between the organizational culture and business performance in star class hotels of Sri Lanka. The study has utilized data collected from 215 senior managers employed in star class hotel sector using self-administered questionnaire. CFA SEM analysis has performed using AMOS 21 to identify the relationship between the variables examined. The quantitative data analysis revealed there is a significant relationship between all four elements of organizational culture and business performance. The organizational culture was reflected through four dimensions organizational climate, flexibility or the support to change, team work and employee empowerment. This study develops a better understanding of elements of organizational culture and their influence on business performance. JEL: D23; L80; L83