In present study, anti-hyperglycemic effect of polyherbal formulation BS019 was assessed using normal hypoglycemic model and Alloxan induced diabetic model. Oral administration of the BS019 at doses of 200 mg/kg and 400 mg/kg showed decreased levels of blood glucose. The effects of BS019 were in dose dependent manner. The results were similar to standard glibenclamide (10mg/kg, p.o). The observed anti-hyperglycemic activity was associated by the phytochemicals which were present in the BS019. The results indicated that BS019 possessed anti- hyperglycemic activity.