Breast cancer is the second most common type of cancer (after lung cancer), and the fifth most common cause of cancer death. This study aimed to assess the blood circulating serum biomarkers for the early diagnosis of breast cancer TNM stage III in local population of Pakistan. This cross sectional study was conducted in health department Punjab during 2019. The main eligibility requirements for this study included the patient's written informed consent, metastatic breast cancer, patients entering first-line chemotherapy (chosen by clinicians) with or without targeted therapy, life expectancy of at least three months, and measurable or evaluable disease. The results indicates that CTC, CEA and ALP are the best indicating serum biomarkers for the diagnosis and progression of breast cancer. Mean, median and SD shows that there is a significant relationship in these serum biomarkers. It is concluded that biomarkers and TAC are the useful tool for the analysis of progression of breast cancer in females.