The phenomenon of drug resistance poses a major problem to a successful chemotherapy. Therefore, we have to look for newer component with effective modes by incorporating them into the test bench. Our purpose was to study the localization site of resin of Globularia alypum (L.). Also, we investigated its antitumor activity against the Hep-2 cell and Vero cell in vitro by the observation under an inverted microscopy. Hep-2 cells and Vero cell were seeded at a density of 105 or 106 cells /mL and cultured in the presence of the various concentrations of the extract for 24 h. A study of the anatomy of G. alypum using double coloration has highlighted the presence of globular trichomes which can be a secretary site of resin. An in vitro cytotoxicity experience showed that the most of Hep-2 cell and Vero cell in monolayer with a density of 106 cells/well were destroyed with concentration of 26 and 13 mg/ml.