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Intermediate_Assemblies.tar.gz: Intermediate genome assemblies e.g. raw wtdbg assembly (CD.raw.fa), polished wtdbg assembly (CD.cns.fa), 1st pilon polished assembly (CDF2_pilon1.fasta), 2nd pilon polished assembly (CDF2_pilon2.fasta) and RNA-scaffolded assembly (CDF2_pilon2_prna.fasta). Folders with run_"assembly name" are BUSCO output for each of the assembly. BRAKER2.tar.gz: BRAKER2 genome annotation output containing the initial set of predicted protein-coding genes as well as training intermediate files. BUSCOv3.tar.gz: BUSCO assessment of publicly available Decapod crustacean genome assemblies Cdes.filtered.codingseq: Filtered set of protein-coding sequences Cdes.filtered.faa: Translation of the filtered protein-coding sequences CDF2.NCBI.fasta.masked.gz: Repeat-masked (softmasked) Cherax destructor genome Cqua_transcriptome.tar.gz: rnaSPAdes output (combined fasta) of all Cherax quadricarinatus transcriptomes and its reduced dataset generated by EvidentialGene. Quast.tar.gz: Quast output of all Decapod crustacean genome assemblies assessed in this study Repeat_Annotation.tar.gz: Repeat annotation (.gff3) based on Cherax destructor-specific de novo repeat library and its summary (.tbl) RepeatLibrary.tar.gz: Cherax destructor-specific de novo repeat library generated by RepeatModeler Wtdbg2_assembly.log: Wtdbg2.5 log file showing exact command used, kmer distribution, memory usage and assembly duration. CAZY_Annotation.tar.gz: dbCAN2 Identification of CAZy in the selected crustacean proteomes as well as list of cellulase-associated GH groups (glycoside hydrolase). Orthofinder.tar.gz: Orthofinder2 output and proteomes of each crustacean used to infer orthologous clustering. GH9_Analysis.tar.gz: Selected GH9-associated protein sequences, amino acid alignment and IQTree output. Cdes_mito.gbf: GenBank file of the annotated complete mitogenome Cdes.filtered.codingseq: Cherax destructor protein-coding genes with homology to other crustacean proteomes based on Orthofinder2 orthologous grouping. Cdes.filtered.faa: Cherax destructor proteins with homology to other crustacean proteomes based on Orthofinder2 orthologous grouping. Cdes.ortholog.list: List of predicted Cherax destructor proteins with homology to other crustacean proteomes based on Orthofinder2 orthologous grouping.
Nanopore, repeat annotation, genome annotation, Illumina, Cherax destructor, de novo assembly, Crayfish
Nanopore, repeat annotation, genome annotation, Illumina, Cherax destructor, de novo assembly, Crayfish
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