Objective: The objective of the study was to evaluate in-vitro efficacy of antibacterial activity of crude methanol, n-hexane, chloroform, ethyl acetate and aqueous extracts of Zizyphus jujuba against some human pathogenic bacterial strains. Z.jujuba commonly called, Red date or Chinese date or Bera (Pushto), belonging to family Rhamnaceae, is used primarily for its fruits. Jujube, a delicious fruit, is an effective herbal remedy improving stamina and muscular strength and aids weight gain, strengthens liver function and increases immune system resistance. Methodology: Crude methanol, n-hexane, chloroform, ethyl acetate and aqueous extracts of Zizyphus jujuba were used for antibacterial screening. Antibacterial activity was tested against pathogenic bacterial strains of Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Salmonella typhi, Bacillus Pumilus, Klebsiella pneumoniae, Enterobacter aerogenes and Streptococcus pneumonia. Antibacterial and antifungal activities of Zizyphus jujuba extract was carried out by using disc diffusion method. Results: The n-hexane and aqueous fractions showed significant activity of 60 and 66.66% against Bacillus pumalis and Pseudomonas aerugenosa, respectively. Activity of ethyl acetate fraction was 65.38, 62.96, 62.96 and 72% against Staphylococcus epidermidis, Salmonella typhi, P. aerugenosa and B. pumalis, respectively. On the contrary, no activity of this fraction was recorded against Streptococcus pneumoniae. The crude methanol, n -hexane, chloroform, ethyl acetate and aqueous fractions showed 41.37, 44.82, 41.37, 55.17 and 44.82% activity against Enterobacter aerogenes respectively. The crude methanolic extract and n-hexane fractions were inactive against Escherichia coli, S. pneumoniae and Klebsella pneumonia respectively. Conclusions: The results concludes that n-hexane, ethyl acetate and aqueous extracts of Zizyphus jujuba possess potent antibacterial activity and further the plant can be explored for isolation of its active constituents.