Biochemical changes in the mycelia mat of Phyllosticta Zingiberi resistant to carbendazim with 21 parameters viz. starch, reducing sugar, total sugar, polyphenol, DNA , RNA,ash, moisture, nitrogen, phosphorus, potassium, calcium, manganese, sulphur, sodium, zinc,, ferrous copper, mangese, molybdenum, and boron were found to be more in resistant isolates than in sensitive isolates. Thirteen Phyllosticta Zingiberi isolates were obtained from leaf spot of Zingiber officinale and showed 2% to 9% minimum Inhibitory Concentration against carbendazim In vitro and In vivo.Infection of Zingiber leaves with resistant and sensitive isolates reduced the contents of all biochemical compounds, due to utilization by fungal pathogen for their growth and metabolism and causes deterioration of the nutrients present in the leaves.