The study aimed at describing and analyzing efforts to prevent human trafficking by the Tapen Bikomi Foundation in Timor Tengah Utara Regency. The Tapen Bikomi Foundation is one of the local NGOs and partners of the regional government of Timor Tengah Utara Regency in various human trafficking prevention activities in the area. Timor Tengah Utara Regency is one district in the province of East Nusa Tenggara, Indonesia. In Timor Tengah Utara Regency, human trafficking is one of the serious problems that has claimed many victims. Trends in human trafficking cases in Timor Tengah Utara Regency occur through illegal recruitment and sending a mode of migrant workers. This study was about the various efforts of the Tapen Bikomi Foundation in preventing the practice of human trafficking in Timor Tengah Utara Regency. Prevention efforts included things done for the community, for the government and together with the government in Timor Tengah Utara Regency. This research used a qualitative descriptive approach. Data obtained through in-depth interviews with informants, observations not involved in the activities of the Tapen Bikomi Foundation and documentation studies. The results showed that in preventing human trafficking in the Timor Tengah Utara Regency, the Tapen Bikomi Foundation carried out three main activities that is, empowering the community, building a network of cooperation with the government and strengthening the capacity and policies of government institutions.