Protection scheme required for the protection of power system components against abnormal conditions such as faults etc., and that essentially consists of protective relaying and circuit breaker. Protective relay senses the fault and determines the location of fault. Then, protective relay sends the tripping command to the circuit breaker. Therefore, proper care should be taken in designing and selecting an appropriate relay which is reliable, efficient and fast in operation. The voltage transformer and current transformer continuously measure the voltage and current of an electrical system and are responsible to give feedback signals to the relays to enable then to detect abnormal conditions. This paper describes differential protection for power transformer, especially the rating of purposed system is 100 MVA, 230 kV 33 kV at substation. Thida Win | Hnin Nandar Maung | Ye Min Hein "Differential Protection of Power Transformer in Substation" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-5 , August 2019, URL: https://www.ijtsrd.com/papers/ijtsrd27995.pdf