Objective- To examine the job of lactobacillus in bacterial vaginosis and vaginal discharge Methodology- For this all patients were selected who had the problem of vaginal discharge and after this patients who had normal discharge through vaginal and who had bacterial vaginosis were separated and on both of these groups the role of lactobacillus was observed. Findings- In this study 270 patients were selected in which 140 patients were suffering the problem of normal vaginal release while remaining 60 of them were suffering in bacterial vaginosis. On both of these groups the role of lactobacillus was thoroughly observed. Patients who had normal release through vaginal 85.7% of them had a count of lactobacillus greater than 106(CFU/mL) while the patients who had bacterial vaginosis, 85% of them had a count of lactobacillus less than 106(CFU/mL). With the help of the test of Fisher’s exact, the increase in lactobacillus count in normal vaginal discharge and decrease in lactobacillus count can be demonstrated (at P value = 0.001) . Conclusion- In this study it can be concluded that there is a relationship of increased count of lactobacillus and decreased count of lactobacillus in normal and bacterial vaginosis respectively. Keywords: Lactic acid; Vaginal discharge; Bacterial vaginosis; Lactobacillus.