The software implementation of iris recognition system introduces in this paper. This system intends to apply for high security required areas. The demand on security is increasing greatly in these years and biometric recognition gradually becomes a hot field of research. Iris recognition is a branch of biometric recognition method. In thesis, Iris recognition system consists of localization of the iris region and generation of data set of iris images followed by iris pattern recognition. In thesis, a fast algorithm is proposed for the localization of the inner and outer boundaries of the iris region. Located iris is extracted from an eye image, and, after normalization and enhancement, it is represented by a data set. Using this data set a Neural Network NN is used for the classification of iris patterns. The adaptive learning strategy is applied for training of the NN. The implementation of the system is developed with MATLAB. The results of simulations illustrate the effectiveness of the neural system in personal identification. Finally, the accuracy of iris recognition system is tested and evaluated with different iris images. Mo Mo Myint Wai | Nyan Phyo Aung | Lwin Lwin Htay "Software Implementation of Iris Recognition System using MATLAB" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-5 , August 2019, URL: https://www.ijtsrd.com/papers/ijtsrd25258.pdf