Background: Chronic kidney disease is a progressive loss in renal function over period of many months or years. There is decline in nephron function and number generally quantitated as reduction in glomerular filtration rate. As the GFR declines there is accumulation of metabolic end products excreted by kidney. Serum lipase is mainly marker of pancreatic & hepatobiliary disease but its level is also high in chronic renal disorder. Lipase is filtered by glomerulus and almost completely reabsorbed and metabolised by renal tubules, thus chronic kidney disease patient have elevated serum lipase level. Our study was aimed to find out the status of serum lipase in non diabetic chronic kidney disease subjects and healthy controls. Materials and Methods: The present study is a case control study, conducted on 100 non diabetic chronic kidney disease patients . Cases were selected from Urology and Medical ward of Jawahar Lal Nehru Medical College and Associated Group of Hospitals, Ajmer. Age and sex matched healthy controls (n = 50) were selected from MOPD of Jawahar Lal Nehru Medical College and Associated Group of Hospitals, Ajmer. The present study is approved by Institutional Ethical Committee. Results: The mean activity of serum lipase was significantly higher in non diabetic chronic kidney disease subjects compared to that in the healthy controls (p < 0.0001). 85 out of 100 non diabetic chronic kidney disease patients have higher serum lipase levels than normal (>70 U/L). Conclusion: Serum lipase level was found to be elevated in non diabetic chronic kidney disease patients. The observations of this study has also revealed that 85 out of 100 non diabetic chronic kidney disease patients have higher serum lipase levels than normal (>70 U/L). Serum lipase as a diagnostic tool in recognizing acute pancreatitis leads to false positive results in chronic kidney disease patients. Serum lipase can be used as a biomarker for the early detection of chronic kidney disease in the general population to prevent the morbidity and mortality which are associated with chronic kidney diseases