Introduction: This study was undertaken to improve patient adherence to lipid tests. More numbers of laboratories are now performing these tests without the need for a 12 h fast at random times during the day.Methods: The study consisted of 51 volunteers and venous blood was collected in a 12 h fast and after a meal the next day. The volunteer then comes back to the laboratory for blood collection 2, 3 and 4 h after having usual breakfast. The following tests: cholesterol, triglycerides, C-LDL, C-HDL and VLDL with the Enzyme/Colorimetric method on Beckman-Coulter®AU5800 equipment and Beckman-Coulter reagent will be performed. In addition to the dosage, C-LDL was calculated by Fried Ewald Equation.Results: It was observed that there was no significant difference for the parameters of TC and C-HDL as compared in the fasting lipid profile versus 2, 3 and 4 h after the meal, and for the calculated C-LDL, with average TC (p=0.237), C-HDL (p=0.130) and for C-LDL (p=0.089). However, for the dosed C-LDL, TG and VLDL showed significant differences with the respective mean concentrations and standard deviation for each hour after 2 h C-LDL (112.1 ± 33.6 mg/dL, p=0.008), 3 h (111.7 ± 35.0 mg/dL, p=0.019) and 4 h (115.0 ± 34.9 mg/dL, p=0.017) for TG 2 h (156.0 ± 86.4 mg/dL, p=0.000), 3 h (148.5 ± 92.0 mg/dL, p=0.000) and 4 h (143.4 ± 93.0 mg/dL, p=0.000) and for VLDL calculated: 2, 3 and 4 h (35.9 ± 53.5 mg/dL, p=0.000, 35.2 ± 53.6 mg/dL, p=0.001, and 34.0 ± 53.6 mg/dL, p=0.000).Conclusion: Our data confirmed that the meal did not influence the TC, C-HDL and C-LDL calculate data, but for TG, VLDL and C-LDL doses a significant difference was observed at post-meal concentrations. Analyzing our data, we observed that the best blood collection time could be between 2 and 3 h after the meal, where the degree of lipemia would have less influence in most individuals.