This project is described in: A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging (2019) and can be found on BioRxiv: https://www.biorxiv.org/content/10.1101/716787v1 and at Nature Methods: https://www.nature.com/articles/s41592-020-0782-3 Here, we provide example raw datasets of the DNA FluoroCubes with six dyes, Single Dye Cubes, one dye double-stranded DNA constructs, single, biotinylated dyes, and Compact Cube used to determine the photophysical properties. In the zip folder you will find the following datasets with raw, example total internal reflection fluorescence (TIRF) microscopy movies: - FluoroCube_Movies which includes the following movies: - ATTO-488_FluoroCube - ATTO-565_FluoroCube - ATTO-647N_FluoroCube - Cy3_FluoroCube - Cy3N_FluoroCube - Cy5_FluoroCube - Single-Dye-Cube_AND_dsDNA_Movies which includes the following movies: - ATTO-488_dsDNA_single-dye - ATTO-488_Single-Dye-Cube_single-dye - ATTO-565_dsDNA_single-dye - ATTO-565_Single-Dye-Cube_single-dye - ATTO-647N_dsDNA_single-dye - ATTO-647N_Single-Dye-Cube_single-dye - Cy3_dsDNA_single-dye - Cy3_Single-Dye-Cube_single-dye - Cy3N_dsDNA_single-dye - Cy3N_Single-Dye-Cube_single-dye - Cy5_dsDNA_single-dye - Cy5_Single-Dye-Cube_single-dye - Single-biotinylated-dye_AND_dsDNA_Movies which includes the following movies: - ATTO-488_dsDNA_single-dye - ATTO-488_single-biotinylated-dye - ATTO-647N_dsDNA_single-dye - ATTO-647N_single-biotinylated-dye - Cy3_dsDNA_single-dye - Cy3_single-biotinylated-dye - Cy3N_Single-Dye-Cube_all-positions_Movies which includes the following movies: - Cy3N_Single-Dye-Cube_SDC-01_single-dye - Cy3N_Single-Dye-Cube_SDC-02_single-dye - Cy3N_Single-Dye-Cube_SDC-03_single-dye - Cy3N_Single-Dye-Cube_SDC-04_single-dye - Cy3N_Single-Dye-Cube_SDC-05_single-dye - Cy3N_Single-Dye-Cube_SDC-06_single-dye - Compact-Cube_Movies which includes the following movies: - Cy3_FluoroCube_six-dyes - Cy3_Single-Dye-Cube_single-dye - Cy3_Compact-Cube_six-dyes - Cy3_Compact-Cube_single-dye