Middle Cerebral Artery Occlusion (MCAO) surgery performed on 129/Sv mice to model a 35-minutes transient focal cerebral ischemia according to a modified version of the Koizumi method. Silicone resin-coated nylon monofilament (MCAO suture, Doccol Corporation, Cat# 702234PK5Re) is inserted through the common carotid artery (CCC). Contact: danielmanriquecastano@gmail.com Reference: Koizumi J, Yoshida Y, Nakazawa T, Ooneda G. Experimental studies of ischemic brain edema. I: A new experimental model of cerebral embolism in rats in which recirculation can be introduced in the ischemic area. Jpn J Stroke 1986(8):1–8.

{"references": ["Koizumi J, Yoshida Y, Nakazawa T, Ooneda G. Experimental studies of ischemic brain edema. I: A new experimental model of cerebral embolism in rats in which recirculation can be introduced in the ischemic area. Jpn J Stroke 1986(8):1\u20138."]}