Background:Polycystic ovary syndrome (PCOS) is a common endocrine condition affecting women of reproductive age and characterized by chronic anovulation, hyperandrogenism, and polycystic ovaries. PCOS patients usually develop thyroid disorders which have an adverse effect on fertility and other hormones. Hence this study was done to estimate levels of TSH , LH, FSH, and Prolactin. Aims &Objectives: To study levels of TSH , LH, FSH, and Prolactin and to compare it with healthy matched controls Materials & Methods: The study was conducted in Department of Biochemistry ,Kurnool Medical College and Department of Obstetrics & Gynaecology , Government General Hospital , Kurnool Results:The PCOS group exhibited significantly higher mean thyroidstimulating hormone level , luteinizing hormone level.The study showed that LH is raised in PCOS with p value of 0.0001 which is statistically significant. The ratio of LH/FSH was more than 2:1in cases. and a non-significantly higher follicle-stimulating hormone level than the control group. Prolactinlevels were normal. Conclusion: The findings of the study showed that PCOS is associated with hypothyroidism and other hormonal imbalance as compared to normal population. So Hormonal dysfunctions in PCOS manifested together or independently. PCOS women can be sub grouped based on clinical features suggestive of endocrinological malfunctions and can be investigated accordingly for selection of appropriate treatment modalities