Within mega-diverse Hymenoptera non-aculeate parasitic wasps represent 75 % of all hymenopteran species. Their ovipositor dual-functionally injects venom and employs eggs into (endoparasitoids) or onto (ectoparasitoids) diverse host species. Few endoparasitoid wasps such as Pimpla turionellae paralyze the host and suppress its immune responses, such as encapsulation and melanization, to guarantee their offspring’s survival. In our proteo-transcriptomic analysis we shed new light on the venom biology of the endoparasitoid Pimpla turionealle. All additional data is made available here, such as the transcriptome assembly file, CDS prediction and all proteome data files including the raw data. All alignments to train HMMsearch and JACKHMMERsearch are stored here as well and the alignments of identified venom proteins (known and novel). The readme file gives further explanation/information.