{"references": ["A. B. Allison J. R. Ballard, R. B. Tesh, J. D. Brown, M. G. Ruder, M. K. Keel, B. A. Munk, R.l M. Mickley, S. E. J. Gibbs, A. P. A. Travassos da Rosa, J. C. Ellis, H. S. Ip, V. I. Shearn-Bochsler, M. B. Rogers, E. Ghedin, E. C. Holmes, C. R. Parrish, C. Dwyerj Cyclic Avian Mass Mortality in the Northeastern United States Is Associated with a Novel Orthomyxovirus.// //J Virol. \u2013 2015. \u2013 \u2116 89 (2). \u2013 P. 1389\u20131403.", "B. Barrett Chapter 18\u2014Viral Upper Respiratory Infection. In: Rakel D., editor. Integrative Medicine (Fourth Edition) Elsevier; Amsterdam, The Netherlands: 2018. Pp. 170\u2013179.e7", "X. Cui, A. Das, A. N. Dhawane, J. Sweeney, X. Zhang, V. Chivukula, S. S. Iyer. Highly specific and rapid glycan based amperometric detection of influenza viruses. Chem. Sci. 2017;8:3628\u20133634. Doi: 10.1039/C6SC03720H", "I. Dukhovlinov, R. Al-Shekhadat,E. Fedorova, L. Stepanova, M. Potapchuk, I. Repko, O. Rusova, A. Orlov, L. Tsybalova, O. Kiselev Study of immunogenicity of recombinant proteins based on hemagglutinin and neuraminidase conservative epitopes of Influenza A virus. //Med Sci Monit Basic Res. \u2013 2013. \u2013 \u2116 19. \u2013 P. 221\u2013227", "E. G. Deeva Influenza. On the verge of a pandemic. Rukovod-stvo dlja vrachey. \u041coskow: GeotarMedia; 2008 (in Russian)", "B. Efron Bootstrap Methods: Another Look at the Jackknife.//B. Efron.//Ann. Statist. \u2013 1979. \u2013 \u2116 7 (1). \u2013 P.1 \u201326", "S. Faccini , A. De Mattia, C. Chiapponi, I. Barbieri, M. B. Boniotti, C. Rosignoli, G. Franzini, A. Moreno, E. Foni, A. D. Nigrelli. Development and evaluation of a new Real-Time RT-PCR assay for detection of proposed influenza D virus. J. Virol. Methods. 2017;243:31\u201334. doi: 10.1016/j.jviromet.2017.01.019", "A. Hushegyi, T. Bertok, P. Damborsky, J. Katrlik, J. Tkac. An ultrasensitive impedimetric glycan biosensor with controlled glycan density for detection of lectins and influenza hemagglutinins. Chem. Commun. 2015;51:7474\u20137477. doi: 10.1039/C5CC00922G", "U. Jarocka, R. Sawicka, A. G\u00f3ra-Sochacka, A. Sirko, W. Dehaen, J. Radecki, H. Radecka. An electrochemical immunosensor based on a 4,4\u2032-thiobisbenzenethiol self-assembled monolayer for the detection of hemagglutinin from avian influenza virus H5N1. Sens. Actuators B Chem. 2016;228:25\u201330. doi: 10.1016/j.snb.2016.01.001", "J. Lui, J. Wu, X. Zeng, D. Guad, L. Zou, L. Yi, L. Liang, H. Ni, M. Kang, X. Zheng, H. Zhong, X. He,C. Monagin, J. Lin, C. Ke. Continuing reassortment leads to the genetic diversity of influenza virus H7N9 in Guangdong, China. J Virol. 2014 Aug;88(15):8297- 306. doi: 10.1128/JVI.00630-14. Epub 2014 May 14", "J. Lui, J. Raghwani J, R. Pryce, T. A. Bowden, J. Th\u0435z\u0435, S. Huang, Y. Song, L. Zou, L. Liang, R. Bai, Y. Jing, P. Zhou, M. Kang, L. Yi, J. Wu, O. G. Pybus, C. Ke. Molecular Evolution, Diversity, and Adaptation of Influenza A(H7N9) Viruses in China. Emerg Infect Dis. 2018 Oct;24(10):1795-1805. doi: 10.3201/eid2410.171063", ". T. T. Lam, et al. Systematic phylogenetic analysis of influenza A virus reveals many novel mosaic genome segments. Infect. Genet. Evol. 2013;18:367\u2013 378.", "D. Liu, et al. Origin and diversity of novel avian influenza A H7N9 viruses causing human infection: phylogenetic, structural, and coalescent analyses. Lancet. 2013;381:1926\u20131932", "R. Manzoor, M. Igarashi, A. Takada. Influenza A Virus M2 Protein: Roles from Ingress to Egress. Int. J. Mol. Sci. 2017;18 doi: 10.3390/ijms18122649", "www.ncbi.nlm.nih.gov", "N. Nagarajan, C. Kingsford. GiRaF: robust, computational identification of influenza reassortments via graph mining./ //Nucleic Acids Research. \u2013 2010. \u2013 P. 1\u201310", "Orthomyxoviridae\u2014Negative Sense RNA Viruses\u2014Negative Sense RNA Viruses. [(accessed on 6 September 2018)];2011 Available online: https://talk.ictvonline.org/ictv-reports/ictv_9th_report/negative-sense-rna-viruses-2011/w/negrna_viruses/209/orthomyxoviridae", "M. Ozawa, Y. Kawaoka. Crosstalk between animal and human influenza viruses./M. Ozawa, Y. Kawaoka//Annu Rev Anim Biosci. \u2013 2013. \u2013 \u2116 1. \u2013 P. 21\u201342", "C. Paules, K. Subbarao. Influenza. Lancet. 2017;390:697\u2013708.doi: 10.1016/S0140- 6736(17)30129-0", "Past Pandemics|Pandemic Influenza (Flu)|CDC.[(accessed on 6 September 2018)]; Available online:https://www.cdc.gov/flu/pandemicresources/basics/past-pandemics.html", "S. Smith, M. Waterman Identification of Common Molecular Subsequences.//Journal of Molecular Biology. \u2013 1981. \u2013 \u2116 147. \u2013 P. 195\u2013197", "C. B. Staats, R. G. Webster, R. J. Webby . Diversity of influenza viruses in swine and the emergence of a novel human pandemic influenza A (H1N1)./ // Influenza Other Respir Viruses. \u2013 2009. \u2013 \u2116 3 (5). \u2013 P. 207\u2013213", "T. Shelby, T. Banerjee, J. Kallu, S. Sulthana, I. Zegar, S. Santra. Novel magnetic relaxation nanosensors: An unparalleled \"spin\" on influenza diagnosis. Nanoscale. 2016;8:19605\u201319613.doi: 10.1039/C6NR05889B.", "Y. \u2013T. Tseng, C.-H. Wang, C. \u2013 P. Chang, G. \u2013 B. Lee. Integrated microfluidic system for rapid detection of influenza H1N1 virus using a sandwichbased aptamer assay. Biosens. Bioelectron. 2016;82:105\u2013111. doi: 10.1016/j.bios.2016.03.073", "N. J. Maclachlan; C. E. Mayo; P. W. Daniels; G. Savini; S. Zientara; E. P. Gibbs. Bluetongue. Rev. Sci. Tech.2015, 34, 329\u2013340", "T. T. Lam, et al. Systematic phylogenetic analysis of influenza A virus reveals many novel mosaic genome segments. Infect. Genet. Evol. 2013;18:367\u2013 378", "R. G. Webster, W. J. Bean, O. T. Gorman, T. M. Chambers, Y. Kawaoka. Evolution and ecology of influenza A viruses.//Microbiol Rev. \u2013 1992. \u2013 \u2116 56 (1). \u2013 P. 152\u2013179", "C. B. Staats, R. G. Webster, R. J. Webby . Diversity of influenza viruses in swine and the emergence of a novel human pandemic influenza A (H1N1)./ // Influenza Other Respir Viruses. \u2013 2009. \u2013 \u2116 3 (5). \u2013 P. 207\u2013213", "D. Liu, et al. Origin and diversity of novel avian influenza A H7N9 viruses causing human infection: phylogenetic, structural, and coalescent analyses. Lancet. 2013;381:1926\u20131932", "K. H. Zhumatov, M. Kh. Sayatov, A. I. Kydyrmanov, A/H7 Flu viruses: disrtibution, genetic variability, pathogeneticy for avian and human.Eurasian Journal of Applied Biotechnology.2013;2:12-16", "Bi Yuhai, Liu Jingyuan, Xiong Haofeng, Zhang Yue, Liu Di, Liu Yingxia, F. George. Gao, and Beibei Wang. A new reassortment of influenza A (H7N9) virus causing human infection in Beijing, 2014. Sci Rep. 2016; 6: 26624", "E. G. Deeva Influenza. On the verge of a pandemic. Rukovod-stvo dlja vrachey. \u041coskow: GeotarMedia; 2008 (in Russian)", "R. G. Webster, T. Ito, J. N.S.S. Couceiro, S. Kelm, L. G. Baum, S. Krauss, M. R. Castrucci, I. Donatelli, H. Kida, J. C. Paulson and Y. Kaawaoka.1998. Molecular basis for the generation in pigs of influenza A viruses with pandemic potential. J.Virol.72:7367- 7373", ". N. L. Varich, A. K. Gitel'man, A. A. Shilov, Iu. A. Smirnov, N. V. Kaverin. Differential incorporation of genomic segments into the influenza A virus reassortants in mixed infection]. Vopr Virusol. 2009 JanFeb;54(1):7-11. Russian", "T. Tada, K. Suzuki, Y. Sakurai, M. Kubo, H. Okada, T. Itoh, K. Tsukamoto. Emergence of Avian Influenza Viruses with Enhanced Transcription Activity by a Single Amino Acid Substitution in the Nucleoprotein during Replication in Chicken Brains.//J Virol. \u2013 2011. \u2013 \u2116 85 (19). \u2013 P.10354\u201310363", "K. Tamura, D. Peterson, N. Peterson. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods//Mol. Biol. Evol. \u2013 2011. \u2013 \u2116 28 (10). \u2013 P. 2731-2739"]}

The influenza virus is a serious pathogens of animals, humans and birds that regularly causes epidemics, as well as high-mortality pandemics; therefore, an analysis of the assessment of reassortment in the hemagglutinin (HA), neurominidase (NA) and nucleoproteine (NP) genes is necessary. Reassortment causes the necessary genetic variability, which allows a virus with high efficiency to overcome the interspecies barrier. Determination of the reassortment events will allow assessing the degree of variability of the genes of the proteins responsible for the infection process in the infection of the cell.Reassortment events in HA, NA and NP genes of H1N1 and H7N9 strains of Influenza A virus were studied. To characterize the reassortant viruses that have obtained the genes for surface (HA, NA) and internal proteins (NP) from the low pathogenic avian influenza virus subtype H7, and the genes for the highly productive human influenza virus H1, the study of post-reassortment interaction of genes, optimization of the gene composition of highly productive reassortants. Materials and methods. The nucleotide sequences of the investigated genes of hemagglutinin, neurominidase, and nucleoproteine proteins for determining the reassortment events were taken from the National Center for Biotechnological Information (NCBI) GenBank (http://www.ncbi.nlm.nih.gov/). The assessment of the reassortment was carried out by aligning nucleotide sequences using the ClustalW algorithm. Phylogenetic analysis was calculated by the method of maximum likelihood. The dendrogram was built using the MEGA6 program. The reliability of the resulting phylogenetic tree was obtained using bootstrap analysis. The level of reassortment was determined by the GiRaF program. Results. It has been shown that events of the reassortment of the HA gene on two clusters are present within and between subtypes H1N1 and H7N9. The reassortment of the NA genes of two subtlety subtypes shows that among them, each is produced by the genes of the reassortment and have sequences of genes of only one subtype of the influenza A virus. These events of the reassortment of the NA genes are present within the subtypes of H1N1 and H7N9, but not between them. It has been established that events of reassortment of NP genes are not present between subtypes H1N1 and H7N9. The absence of reassortment events in NA, NA and NP genes of the analyzed strains was shown. Conclusions. The assortment events of the HA, NA, and NA genes in subtypes of the influenza A H1N1 and H7N9 viruses show the presence of subtypes inside and not between them. This suggests that genetic polymorphism should be investigated in the subtypes by the definition of mutations, with further evaluation of the variability of the genetic markers of the genes of the proteins studied. Consequently, the absence of reassortment events in HA, NA, and NP genes of the Influenza A strain H1N1 and H7N9 was shown. The results of this research confirm previously conducted studies and explain the evolution of NA, NA and NP genes of the Influenza A virus.