Objective: To explore the application effect of Quill suture in perineal wound of puerpera. Methods: A total of 110 parturients who wish to give birth naturally from January 2018 to August 2019 were randomly divided into two groups, including observation group (case = 55) and control group (case = 55). In the observation group, 55 parturients who underwent lateral perineal incision and laceration were treated with Quill suture for cosmetic perineal suture; in the control group, 55 parturients who underwent lateral perineal incision and laceration were treated with 2-0 absorbable micro-arbor suture for routine suture. Results: The VAS scores of the observation group at 24 and 48 hours after lateral perineal incision and laceration suture were significantly lower than those of the control group, with statistical significance P < 0.05. The healing time of incision in the observation group was significantly shorter than that in the control group, and the difference was statistically significant (P < 0.05). The healing rate of incision grade A in the observation group was significantly higher than that in the control group, and the difference was statistically significant (P < 0.05). The incision inflammatory reaction rates of the observation group and the control group were (2.22%) and (13.16%) respectively, with statistical significance P < 0.05. Conclusion: Quill suture can effectively relieve the pain of puerpera after lateral perineal incision and laceration suture, improve wound healing and reduce the incidence of wound inflammatory reaction. It has high clinical application value.