Skeletal muscle activity of Fenugreek seeds extract and Fenugreek leaves extract were studied in the green frog (Rana hexadactyla) by the rectus abdominis muscle preparation. Fenugreek seeds extract and Fenugreek leaves extract with distilled water 5μg/ml, 50μg/ml and 500μg/ml concentrations. The result indicated that the treatment of Fenugreek seeds extract and Fenugreek leaves extract alone and combination with acetylcholine produce skeletal muscle activity. Thus from the present study it was concluded that Fenugreek seeds extract and Fenugreek leaves extract were have good skeletal muscle activity alone and combination with Acetylcholine. Fenugreek leaves extract have more potent skeletal muscle activity than Fenugreek seeds extract. Keywords: Skeletal muscle activity, Fenugreek seeds extract and Fenugreek leaves extract Rana hexadactyla, Acetylcholine.