The validated method (MA039) used in France to detect Xylella fastidiosa in plant samples in the framework of regulated surveillance is based on a Real-Time PCR (Harper et al., 2010) applied on plant DNA extracted with the QuickPick SML kit (Bio-Nobile). The MLST (Multi Locus Sequence Typing) (Yuan et al., 2010, EPPO PM7/24) scheme is applied to identify the sequence types and the subspecies. Until now the bacterium X. fastidiosa subspecies multiplex was mostly detected on about forty plant species, mainly ornamental of the Mediterranean area. These methods were optimized to improve their sensitivity on plant matrices such as olive tree, oak and on the insect vector Philaenus spumarius. They should contribute to the revision of the EPPO protocol.