The broad fish tapeworm Dibothriocephalus latus (syn. Diphyllobothrium latum) is one of the most common causative agents of human diphyllobothriosis, a significant fish-borne parasitic zoonosis. In Europe, the occurrence of D. latus has been repeatedly reported in lakes of the Alps region, the Baltic region, Fennoscandia and Russia. Regular detection of D. latus plerocercoids in fish coming from different subalpine lakes linked with ongoing D. latus infection in humans indicates that Alpine region is a rather specific area from the medical, epidemiological and ecological point of view. Results from the examination of 688 European perch (Perca fluviatilis) from six subalpine lakes in Switzerland, France and northern Italy (Lakes Geneva, Neuchâtel, Biel, Como, Maggiore and Iseo) confirmed the ongoing occurrence of D. latus in the Alps region. The detected prevalence of D. latus in the studied Alpine lakes (2% in Lake Neuchâtel; 37.5% in Lake Biel; 6.4% in Lake Geneva; 22.8% in Lake Iseo [2018]; 12.8% in Lake Iseo [2017]; 15.2% in Lake Como; 16.7% in Lake Maggiore) was compared with previously published data. In addition, the importance of the Alpine lakes region and data on the epidemiology and ecology of D. latus related to subalpine lakes were discussed.