Hyperbaric Oxygen (HBO2) Therapy has been used for several decades as an adjunctive treatment to standard care for chronic non-healing wounds. However, very little is known about the factors that influenced physicians use of this mode of therapy in their practice. 1st: To assess Physicians Knowledge, Attitude and Practice of HBO2 Therapy in the treatment of chronic non-healing wounds among physicians, whose practice included wound care, in the Saudi Armed Forces Hospitals during the year 2008. 2nd: To identify the factors associated with their Knowledge, Attitude and Practice of HBO2 Therapy. 110 Physicians participated in a Cross sectional study with an analytical component. Data collection tool was 21-item questionnaire. All of the 110 physicians participated in the study. The results showed that Practice of HBO2 Therapy was adopted by only a minority (7.3%; 95% C. I = 3.7 – 13.7) of Physicians who were concentrated in the eastern province. This was despite that the majority of physicians had a high Knowledge (76%; 95% C.I = 76.4–83.5) and agreed (61.9%; 95% C.I = 52.4–70.6) with the use of HBO2 Therapy. Physicians Knowledge was significantly associated with the dependence on various sources of information to stay informed about the evidence of wound care treatment. Physicians Attitude towards HBO2 Therapy was significantly associated with their Knowledge (P <0.05). Physicians Practice of HBO2 Therapy was significantly associated with Patients voluntary request of HBO2 Therapy (P= 0.006), Symposia was a source of information to stay informed about the evidence of wound care treatments (P= 0.007). The low level of Physicians Practice, in contrast to their high Knowledge of and Attitude towards, HBO2 Therapy in the treatment of chronic non-healing wounds, in the Saudi Armed Forces Hospitals, was mainly due to the unavailability (except in Jubail) and inaccessibility of HBO2 Therapy despite the availability of multiplace HBO2 Chambers. It is suggested that Policy procedures and protocols for use of (or referral for) HBO2 Therapy to be implemented. Also educating both Patients and Physicians and focusing on cost-effectiveness most probably will encourage future use of HBO2 Therapy.