Batig V. M. The effect of omega-3 PUFA Liposan-3 on the content of essential fatty acids in lipid serum of rats with avitaminosis F. Journal of Education, Health and Sport. 2019;9(8):998-1006. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.3418924 http://ojs.ukw.edu.pl/index.php/johs/article/view/7472 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 1223 (26/01/2017). 1223 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Authors 2019; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 16.08.2019. Revised: 22.08.2019. Accepted: 30.08.2019. UDK 616.31:615.015:577.16:612.397 THE EFFECT OF OMEGA-3 PUFA LIPOSAN-3 ON THE CONTENT OF ESSENTIAL FATTY ACIDS IN LIPID SERUM OF RATS WITH AVITAMINOSIS F V. M. Batig Bukovina State Medical University, Chernovtsy flavan.ua@gmail.com Abstract Background. Determine the effect of the drug "Liposan-3" on the content of PUFA in the serum lipids of rats receiving a fat-free diet. Methods. Used the drug "Liposan-3α" containing 14% PUFA with a ratio of ω-6/ω-3 of 0.49. Rats were fed a semi-synthetic fat-free diet for 21 days (group 1), supplemented with 0.5 % liposan (group 2) or 1.0 % liposan (group 3). Controlled changes in live weight and feed intake. After euthanasia, the content of triglycerides, total cholesterol was determined in the blood serum, and the content of PUFA was determined in the fractions of neutral lipids (triglycerides + cholesterol esters) and free fatty acids. In addition, the content of malondialdehyde (MDA), the activity of catalase, and the antioxidant-prooxidant API index were determined in their blood serum. Results. The actual liposan intake was 86.8 mg/kg per day, in terms of the amount of PUFA 11.9 mg/kg, including 8.1 mg/kg ω-3 PUFA. The animal weight gain increased by 30 %, the content of TG and cholesterol remained within the normal range, there was no increase in the content of MDA, the activity of catalase did not decrease, and the API index increased. The content of ω-6 PUFA decreased in both fractions of serum lipids by 19-22 %, however, the containment of ω-3 PUFA increased by 2.5-3 times, while the ratio of ω-6/ω-3 PUFA decreased by 2.3- 3.3 times. Conclusion. The drug "Liposan-3" in doses of 80-130 mg/kg increases the content in blood serum lipids of ω-3 PUFA and normalizes the ratio of ω-6/ω-3 of PUFA. Liposan-3 does not increase lipid peroxidation and has a stimulating effect on the growth of animals. Key words: fatty nutrition, essential fatty acids, ω-6 and ω-3 PUFAs, lipid peroxidation, PUFA preparation.