Data and scripts associated with the manuscript entitled "Spatial reconstruction of single enterocytes uncovers broad zonation along the intestinal villus axis". Files: table_A_LCM_TPM_values.tsv: Gene expression levels of microdissected villus quintiles. First column is the ensemble gene id. Next 15 columns are the raw Kallisto TPM values for villus segments 1 (bottom) to 5 (top) for three different mice (a to c). Additional columns include the external gene name, description, and gene biotype. table_B_scRNAseq_UMI_counts.tsv: Raw UMI counts of cells that were utilized in this study. Analysis is based on raw data from the NCBI GEO datasets GSM2644349 and GSM2644350. Each of the columns represents a single cell, column headers are the corresponding cell barcodes and enable retrieval of tSNE coordinates from table_C_scRNAseq_tsne_coordinates_zones.tsv. Values represent raw UMI counts. table_C_scRNAseq_tsne_coordinates_zones.tsv: tSNE coordinates and reconstructed zones of cells that were utilized in this study. Tab separated text file. Analysis is based on raw data from the NCBI GEO datasets GSM2644349 and GSM2644350. Columns: cell_id: cell barcode, corresponds to column header of Table S2. Seurat tSNE coordinate 1 and tSNE coordinate 2. Last column is the inferred zone (Crypt, V1..V6). table_D_zonation_reconstruction.tsv: Zonation table of reconstructed scRNAseq data. Tab separated text file. Columns: Gene names: gene id, mean expression in each of the crypt zone and 6 villus zones, standard error of the means in the Crypt zone and 6 villus zones, p-value and q-value for the zonation profiles. Script.zip: A zip file that includes the Matlab code for villus zonation reconstruction (villus_zonation_reconstruction.m) and input files.