Supplemental Fig. S1 (related to Fig. 2). Mapping and detection of sensory neurons in human pancreatic duct lesion formation. Supplemental Table S1. Summary of color codes presented in illustrations. Supplemental Table S2. Summary of primary and secondary antibodies used in illustrations. Supplemental Video Legends Supplemental Video S1 (related to Fig. 2) Human pancreatic substance P+ sensory (afferent) nerves. Left (still image): projection of pancreatic innervation at the acinar-islet boundary. Blue: glucagon (islet). Green: neuroendocrine marker PGP9.5 (nerve and islet). Red: substance P (SP, sensory nerve). Sensory nerves appear as yellow fibers (overlap of red SP and green PGP9.5 signals). Broken arrow: enlarged area (right panel). Right: in-depth recording of peri-ductal sensory nerves. Sensory nerves (yellow) are around the islet but not penetrating into the core. White: nuclei. Note that a portion of islet cells are SP dim. The still image and recording were derived from a normal human pancreas (female/age, 51 years/BMI, 20). Supplemental Video S2 (related to Fig. 2 and Supplemental Fig. S1) Mapping and detection of immunoreactive substance P+ neuron in duct lesion formation. Left (still image): tissue map of human pancreas distal to ductal adenocarcinoma. Acinar atrophy is apparent in this condition. Markers: nuclei, white; PGP9.5, green; substance P (SP), red. Broken arrow: intra-pancreatic ganglion (right panel). Right: in-depth recording of immunoreactive SP+ neuron in ganglion. The nucleus (00:04) and the SP+ varicosities are prominently seen. The latter contacts the surrounding SP- neurons. The still image and recording were derived from an area 5-cm distal to the pancreatic ductal adenocarcinoma (male/age, 77 years/staging, T1N0). Supplemental Video S3 (related to Fig. 3) Mouse pancreatic substance P+ sensory (afferent) nerves. Left (still image): projection of pancreatic sensory innervation. In mice, the SP+ sensory nerves follow the arteriole to the islet mantle and penetrating into the core (enlarged in right panel). Markers: blood vessels, blue; PGP9.5, green; substance P (SP), red. Right: in-depth recording of sensory innervation of islet. Unlike the human pancreatic sensory innervation, the mouse SP+ sensory nerves (varicosities) are inside the islet and associate with the peri-islet ganglion. White: nuclear staining and tissue autofluorescence. The still image and recording were derived from a B6 mouse pancreas (male/age, 12 weeks).