Escherichia coli is a fecal-coliformic bacteria that usually uses as bio-indicator in water quality based on biological parameter. Water source which contaminated with E. coli indicates the contamination of human or mammals feces. This study was aimed to analyze the quality of drinking water stations distributed in Pasuruan Regency. The water samples were collected from the drinking water stations of 24 districts in Pasuruan. The biological parameter of water samples were assayed to detect the coliformic bacteria. Water quality was determined as poor and good quality according to drinking water standard in Indonesia. As a result, most of the drinking water stations from 24 districts in Pasuruan Regency had poor water quality. This happened due to the open defecation behaviour of the social community and lack of sanitation. However, the contamination of coliformic bacteria including E. coli can be reduced by socialization to decrease open defecation behaviour including other behaviour which may decrease the hygiene of environment as well as the drinking water stations. Further, strategy development to get healthy environment is needed.