Poster presentation from Experimental Biology 2019. Full text of abstract found at doi: 10.1096/fasebj.2019.33.1_supplement.525.7 Introduction: Alterations in microvessel networks, including angiogenesis and capillary regression, play key roles in disease, injury, and development. Imaging of microvascular networks can capture their spatial structures, but effective study of network architecture requires methods to accurately quantify them. We present REAVER (Rapid Editable Analysis of Vessel Elements Routine), a tool to analyze blood vessel networks, and demonstrate that REAVER has superior performance compared to the state-of-the-art.