A simple, Accurate, precise technique was produced for the simultaneous estimation of the estimation of the Glecaprevir & Pibrentasvir in Pharmaceutical dosage form. Chromatogram was run through Kromosil C18 250x4.6mm,5m. MP containing Buffer 0.01N KH2PO4: ACN taken in the ratio 55:45 was pumped through column at a stream rate of 1ml/min. Buffer utilized in this technique was 0.1% Ortho phosphoric acid buffer. Temperature was maintained at 30°C. Improved wavelength chose was 260 nm. Retention time of Glecaprevir and Pibrentasvir were observed to be 2.501 min and 3.113 min. %RSD of the Glecaprevir and Pibrentasvir were and found to be 0.2 and 0.6 correspondingly. %Recovery was obtained as 99.48% and 99.57% for Glecaprevir & Pibrentasvir correspondingly. LOD, LOQ values get from regression equations of Glecaprevir & Pibrentasvir were 0.71, 2.15 and 0.32, 0.96 respectively. Regression equation of Glecaprevir is y = 8171.x + 4225., y = 8748.x + 1373 of Pibrentasvir. Upkeep times were lessened and run time was reduced, so the procedure made was direct and mild that can be grasped in typical Quality control test in Industries. Key Words: Glecaprevir, Pibrentasvir, RP-HPLC.