These data combine fish sampling, basic physical variables, and macroinvertebrate data collected in main water bodies of Station de biologie des Laurentides (SBL) during BIO2476 field course in September 2018. BIO2476 is an undergrad course in the Department of biological sciences of Université de Montréal. During the field course, students sample fish in main SBL waterbodies using different capture techniques (e.g. electrofishing, minnow traps, fyke nets) and visual observation (snorkeling surveys). Fish counts obtained by these different techniques can be expressed as relative abundances using sampling gear dimensions, duration of sampling and/or water volume covered by sampling gear. Data are collected by participating students using standardized and documented methods under the supervision of HQP (teaching assistants and course lecturer). These data are used by students to compute different ichtyological metrics and write their final report. These data consist mainly in datatables accessible openly but fish scale samples and fish photos are also available upon request.